Journal: Microbiology Spectrum

Article Title: Diagnosis and Treatment of Bacterial Pneumonia in Critically Ill Patients with COVID-19 Using a Multiplex PCR Assay: A Large Italian Hospital’s Five-Month Experience

doi: 10.1128/Spectrum.00695-21

Figure Lengend Snippet: Comparison between the FilmArray pneumonia plus panel and standard-of-care reference testing results for LRT samples from COVID-19 patients with bacterial pneumonia ^a

Article Snippet: In September 2020, when the second wave of the SARS-CoV-2 pandemic was raging in Italy, we implemented the multiplexed, semiquantitative FilmArray pneumonia plus (FA-PP) panel (BioFire, Salt Lake City, UT, USA) assay ( ) for routine clinical use at our institution (a large Italian university hospital).

Techniques: Comparison

Journal: Microbiology Spectrum

Article Title: Diagnosis and Treatment of Bacterial Pneumonia in Critically Ill Patients with COVID-19 Using a Multiplex PCR Assay: A Large Italian Hospital’s Five-Month Experience

doi: 10.1128/Spectrum.00695-21

Figure Lengend Snippet: Quantitative result agreement between the FilmArray pneumonia plus (FA-PP) panel and standard-of-care (SoC) culture testing methods for 202 bacterial organisms detected in bronchoalveolar lavage (BAL) fluid or endotracheal aspirate (ETA) samples from ICU patients. All but 22 (which did not grow in culture) organisms were detected at or above the 1 × 10 4 CFU/ml (BAL fluid sample) or 1 × 10 5 CFU/ml (ETA sample) thresholds for clinically relevant quantification by both methods. The diagonal dashed line connects dots that represent bacterial organisms with fully concordant results (71/202; 35.1%), whereas dots above or below the line represent bacterial organisms with SoC culture loads that exceeded (18/202, 8.9%) or did not exceed (113/202, 56.0%), respectively, those of the FA-PP panel by ≥1 log 10 . Shades of gray are used to depict the different numbers of samples corresponding to each dot.

Article Snippet: In September 2020, when the second wave of the SARS-CoV-2 pandemic was raging in Italy, we implemented the multiplexed, semiquantitative FilmArray pneumonia plus (FA-PP) panel (BioFire, Salt Lake City, UT, USA) assay ( ) for routine clinical use at our institution (a large Italian university hospital).

Techniques:

Journal: Microbiology Spectrum

Article Title: Diagnosis and Treatment of Bacterial Pneumonia in Critically Ill Patients with COVID-19 Using a Multiplex PCR Assay: A Large Italian Hospital’s Five-Month Experience

doi: 10.1128/Spectrum.00695-21

Figure Lengend Snippet: Overview of antimicrobial therapy interventions performed according to FilmArray pneumonia plus (FA-PP) panel results. Interventions were stratified into three relevant groups based on the full concordance of positive (A) or negative (B) FA-PP testing results or the partial or full discordance (C) of FA-PP testing results, respectively, with the results by standard-of-care (SoC) culture for the lower respiratory tract (LRT) samples tested. For each LRT sample, the type(s) of interventions and the antibiotics involved (one or more per intervention) are reported, together with whether the interventions resulted in appropriate antimicrobial escalations/initiations (93 in panel A and 22 in panel C) or deescalations (10 in panel A and 6 in panel C), appropriate antimicrobial discontinuations (5 in panel B), and inappropriate antimicrobial escalations/initiations (4 in panel C). Cases with no antimicrobial therapy interventions (5 in panel A, 85 in panel B, and 4 in panel C) are reported as well.

Article Snippet: In September 2020, when the second wave of the SARS-CoV-2 pandemic was raging in Italy, we implemented the multiplexed, semiquantitative FilmArray pneumonia plus (FA-PP) panel (BioFire, Salt Lake City, UT, USA) assay ( ) for routine clinical use at our institution (a large Italian university hospital).

Techniques:

Journal: Microbiology Spectrum

Article Title: Diagnosis and Treatment of Bacterial Pneumonia in Critically Ill Patients with COVID-19 Using a Multiplex PCR Assay: A Large Italian Hospital’s Five-Month Experience

doi: 10.1128/Spectrum.00695-21

Figure Lengend Snippet: Characteristics of bacterial pneumonia episodes detected or not detected by the FilmArray pneumonia plus panel in 97 mechanically ventilated patients with COVID-19 ^a

Article Snippet: In September 2020, when the second wave of the SARS-CoV-2 pandemic was raging in Italy, we implemented the multiplexed, semiquantitative FilmArray pneumonia plus (FA-PP) panel (BioFire, Salt Lake City, UT, USA) assay ( ) for routine clinical use at our institution (a large Italian university hospital).

Techniques: